(DOC) Gram Negative Unknown Lab Report # 31 | Diana Monroe - zellebrss.tk

 

unknown organism lab report

Nov 23,  · Microbiology Unknown Lab Report Essay Words | 10 Pages. LabMedical Microbiology- Part1– Differential Media/Biochemical Tests, Sp (Set all of your margins to ½”) Purpose: The purpose of this lab is to help you become a little familiar with some of the tests that can be typically performed in a clinical or research lab facility. Dec 03,  · UNKNOWN LAB REPORT. Unknown number Clare Zilch. December 3, General Microbiology INTRODUCTION. This study shows the importance of identifying unknown microorganisms by applying all the methods and techniques that were studied in the microbiology course throughout the semester. Gram Negative Unknown Lab Report # The ability to adapt to a broad range of habitats helps to explain why prokaryotes are the most abundant organism on earth. The main characteristics of a prokaryote include, no nucleus, circular DNA, and no membrane bound organelles. A key feature of nearly all prokaryotic cells is the cell wall, which.


How to Write Microbiology Unknown Lab Report | Example Paper


The way bacteria function and flourish is important, especially for people entering the Healthcare field, because it allows people to have a better understanding on how to work with or fight the bacteria. The General Microbiology I course taught students about many kinds of bacteria.

Multiple procedures were practiced in the Microbiology lab to help students better understand how bacteria thrive, grow, and regenerate. These methods were used to determine which two unknown bacteria were present in the test tube. The professor assigned the Unknown test tube. Unknown organism lab report, an Alternative 4 tube was given unknown organism lab report the Unknown tube was not allowing the gram negative to be shown on the nutrient agar. The procedures from the laboratory manual by McDonald were used to accomplish the tests needed to find the two unknown bacteria 3.

First, the bacteria mix inside the Unknown tube was streaked across a nutrient agar plate using the quadrant streak technique. The quadrant streak helped spread out the bacteria and made it easier for the two different bacteria to make colonies that could be differentiated by the naked eye.

The results were checked to see if two separate bacteria were growing. It looked over grown, so the same nutrient agar test was carried out several times. The results showed the nutrient agar was only allowing one bacteria to grow.

A gram stain was taken from a sample of the bacteria growing on the nutrient agar. The gram stain turned out to unknown organism lab report a positive rod gram stain.

The professor gave an Alternate 4 tube, so the gram negative unknown could be found. A quadrant streak technique was used on a nutrient agar plate using the Alternative 4. A sample of the bacteria growing on the nutrient agar was taken and used for gram stain test, unknown organism lab report.

The Alternative 4 was a gram negative rod. Table I and Flowchart I list all the tests, purposes, results, and order the tests where done for the Gram positive bacteria using unknown organism lab report Unknown tube. Table II and Flowchart II list all the tests, purposes, results, and order the tests where done for the Gram negative bacteria using the Alternative 4 tube.

Enterococcus faecalis. The correct identification for the gram positive bacterium was Bacillus cereus. The B. Originally, a sample from the Unknown tube was supposed to be inoculated on a nutrient agar plate using the quadrant streak technique. This was to allow the gram positive and gram negative to be spread out, creating separate colonies. This never occurred. Several nutrient agar plates where inoculated and two different bacteria never grew.

Then, it was concluded that only one bacterium was growing on the nutrient agar, so a gram stain was performed. A gram positive rod was the outcome, ruling out the gram positive cocci. The Methyl Red test concluded positive for the presence of glucose fermenters. The Casein test concluded negative for the bacterium having casease to hydrolyze casein.

This was an incorrect finding because B. The finding could have been incorrect because the clearing around the bacterium might not have been as clear for the tester to notice, unknown organism lab report. The Glycerol and Maltose test showed fermentation for carbohydrates by changing the solution from yellow to red.

The Maltose test showed positive, but the Glycerol test show incorrect results for negative. The two tests call for the bacterium to be in a liquid form when added to the test tubes, but the test was done from the bacterium being on an agar jell.

The bacterium might not have mixed well with the Glycerol solution, creating an improper answer. Lastly, an Oxidase test showed positive for the bacterium having cytochrome c. This completed the search for the Gram positive rod: B, unknown organism lab report. Since the original Unknown test tube was not allowing the gram negative to grow, an Alternative 4 tube was assigned as well. That test tube only had a gram negative bacterium in it.

The Unknown organism lab report 4 tube was used to inoculate a nutrient agar plate using the quadrant streak technique. The gram negative bacterium grew on the agar and a gram stain was carried out. The gram stain showed a gram negative rod was present. Next, the MacConkey and EMB tests were performed to detect if the bacterium was an enteric and fermenter of lactose. They were positive and acidic. The EMB test produced green metallic colonies, which was a big flag that the bacterium might be Escherichia coli.

The Methyl Red test was positive for the presence of glucose fermentation. The Simmons Citrate showed the bacterium cannot solely use citrate as source of carbon. The last test performed was the Urea test. The negative results explain that the bacterium did not have the enzyme urease, which breaks down urea. The bacterium did not produce an alkaline pH, but an acidic one. All of the test results came out correct, matching all the characteristic of E.

Theodor Escherich, a German bacteriologist and pediatrician, unknown organism lab report, first discovered E. The bacterium was named after him since he isolated and found its components 2. It is most commonly found in mammals intestines. The E. The strain OH7 releases a toxin into the body cavities that initiates an illness in mammals 2.

The number one thing is to unknown organism lab report your hands. Always wash unknown organism lab report hands after going to the bathroom, before working with food, and after coming in contact with animals. Try to avoid unpasteurized dairy products. Always wash vegetables and fruits before eating or preparing them. When cooking meat, use a thermometer to make sure the recommended temperature has been reached to ensure bacteria no longer existed in the food 1.

Killing E. They are easily killed by boiling the water they may be living in or using a basic sterilization process 4. Coli Escherichia Coli. Centers for Disease Control and Prevention, 29 Mar. Unknown organism lab report, Virginia. Thoele, Mary. Salsiver, unknown organism lab report, Bill. Gero, Susie. Lab Manual for General. BIO Louis Community College at Meramec, April Tortora, Gerard J.

Funke, Berdell R, unknown organism lab report. Case, Christine L. Microbiology: An Introduction. Post Your Comments Click here to cancel reply.

 

Example of Unknown Lab Report for Microbiology

 

unknown organism lab report

 

HOW TO WRITE AN UNKNOWN LAB REPORT IN MICROBIOLOGY UNKNOWN LAB REPORT # 1 After performing the Gram stain to determine that the unknown was a Gram negative rod, the organism was grown on a TSA slant for use in inoculating the rest of the biochemical tests. All of the. Nov 23,  · Microbiology Unknown Lab Report Essay Words | 10 Pages. LabMedical Microbiology- Part1– Differential Media/Biochemical Tests, Sp (Set all of your margins to ½”) Purpose: The purpose of this lab is to help you become a little familiar with some of the tests that can be typically performed in a clinical or research lab facility. Dec 03,  · UNKNOWN LAB REPORT. Unknown number Clare Zilch. December 3, General Microbiology INTRODUCTION. This study shows the importance of identifying unknown microorganisms by applying all the methods and techniques that were studied in the microbiology course throughout the semester.